Journal: Thoracic Cancer

Article Title: Circ_0001667 accelerates breast cancer proliferation and angiogenesis through regulating CXCL10 expression by sponging miR ‐6838‐5p

doi: 10.1111/1759-7714.14820

Figure Lengend Snippet: Knockdown of circ_0001667 inhibited proliferation and angiogenesis in breast cancer cells. MDA‐MB‐231 and BT549 cells were transfected with si‐NC or si‐circ_0001667. (a) qRT‐PCR assay for silencing efficiency of si‐circ_0001667. (b‐d) CCK8 and EdU assays to detect proliferation of breast cancer cells. Scale bar: 50 μm. (e and f) Flow cytometry was used to determine the cycle distribution of breast cancer cells. (g) Colony formation assay was used to assess the colony formation ability. (h) Western blot to measure the protein levels of PCNA and Ki67. (i) Tube formation assay was used to analyze angiogenesis of breast cancer cells. (j) Western blot showing the expression levels of VEGFA and FGF2 in breast cancer cells. * p < 0.05.

Article Snippet: The separated proteins were electrotransferred to PVDF membrane (Beyotime), closed with 10% skimmed milk, and incubated with primary antibodies (Abcam) against PCNA (ab18197, 1:1000), Ki67 (ab254123, 1:5000), VEGFA (ab46154, 1:2000), FGF2 (ab208687, 1:1000), CXCL10 (ab9807, 1:2000), or β‐actin (ab6276, 1:5000).

Techniques: Knockdown, Transfection, Quantitative RT-PCR, Flow Cytometry, Colony Assay, Western Blot, Tube Formation Assay, Expressing

Journal: Thoracic Cancer

Article Title: Circ_0001667 accelerates breast cancer proliferation and angiogenesis through regulating CXCL10 expression by sponging miR ‐6838‐5p

doi: 10.1111/1759-7714.14820

Figure Lengend Snippet: Downregulation of miR‐6838‐5p attenuated the effect of circ_0001667 knockdown on cell proliferation and angiogenesis. (a) qRT‐PCR analysis of miR‐6838‐5p levels in cells transfect with anti‐miR‐6838‐5p or anti‐miR‐NC. (b‐j) MDA‐MB‐231 and BT549 cells were transfected with si‐NC, si‐circ_0001667, si‐circ_0001667 + anti‐miR‐NC or si‐circ_0001667 + anti‐miR‐6838‐5p. (b‐d) CCK‐8 and EdU assays detected cell proliferation. (e and f) Flow cytometry determined cell cycle distribution. (g) The colony formation assay assessed the colony formation ability. (h) Western blot measured the protein levels of PCNA and Ki67. (i) Tube formation test analyzed angiogenesis. (j) Western blot showed the expression levels of VEGFA and FGF2. * p < 0.05.

Article Snippet: The separated proteins were electrotransferred to PVDF membrane (Beyotime), closed with 10% skimmed milk, and incubated with primary antibodies (Abcam) against PCNA (ab18197, 1:1000), Ki67 (ab254123, 1:5000), VEGFA (ab46154, 1:2000), FGF2 (ab208687, 1:1000), CXCL10 (ab9807, 1:2000), or β‐actin (ab6276, 1:5000).

Techniques: Knockdown, Quantitative RT-PCR, Transfection, CCK-8 Assay, Flow Cytometry, Colony Assay, Western Blot, Expressing

Journal: Thoracic Cancer

Article Title: Circ_0001667 accelerates breast cancer proliferation and angiogenesis through regulating CXCL10 expression by sponging miR ‐6838‐5p

doi: 10.1111/1759-7714.14820

Figure Lengend Snippet: Overexpression of CXCL10 reversed the effects of miR‐6838‐5p overexpression on cell proliferation and angiogenesis. (a and b) Validation of CXCL10 overexpression by qRT‐PCR and western blot. (c–k) MDA‐MB‐231 and BT549 cells were transfected with miR‐NC, miR‐6838‐5p, miR‐6838‐5p + pcDNA or miR‐6838‐5p + CXCL10. (c–h) CCK8 assay, EdU assay, flow cytometry and colony formation assay detected cell proliferation. (i) Western blot analysis for PCNA and Ki67 levels. (j) Tube formation test detected angiogenesis. (k) Western blotting quantified the levels of VEGFA and FGF2. * p < 0.05.

Article Snippet: The separated proteins were electrotransferred to PVDF membrane (Beyotime), closed with 10% skimmed milk, and incubated with primary antibodies (Abcam) against PCNA (ab18197, 1:1000), Ki67 (ab254123, 1:5000), VEGFA (ab46154, 1:2000), FGF2 (ab208687, 1:1000), CXCL10 (ab9807, 1:2000), or β‐actin (ab6276, 1:5000).

Techniques: Over Expression, Biomarker Discovery, Quantitative RT-PCR, Western Blot, Transfection, CCK-8 Assay, EdU Assay, Flow Cytometry, Colony Assay

Journal: Molecular Neurobiology

Article Title: High Expression of Nicotinamide N -Methyltransferase in Patients with Sporadic Alzheimer’s Disease

doi: 10.1007/s12035-020-02259-9

Figure Lengend Snippet: Primary and secondary antibodies used for Western blotting, immunohistochemistry and confocal microscopy

Article Snippet: Tubulin , 1:1000 , - , ab180207 (Abcam) , 1:5000 (A4416, Sigma) , -.

Techniques: Western Blot, Immunohistochemistry, Microscopy, Confocal Microscopy

Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

Article Title: Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests

doi: 10.1093/cid/cix672

Figure Lengend Snippet: Results of Enzyme-Linked Immunosorbent Assays in Different Serum/Plasma Panels

Article Snippet: Purified DENV1-NS1 protein was purchased from the Native Antigen (United Kingdom).

Techniques:

Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

Article Title: Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests

doi: 10.1093/cid/cix672

Figure Lengend Snippet: Sensitivity and Specificity of Enzyme-Linked Immunosorbent Assays

Article Snippet: Purified DENV1-NS1 protein was purchased from the Native Antigen (United Kingdom).

Techniques:

Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

Article Title: Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests

doi: 10.1093/cid/cix672

Figure Lengend Snippet: Results of nonstructural protein 1 (NS1) immunoglobulin G (IgG) enzyme-linked immunosorbent assays (ELISAs) for secondary dengue virus (sDENV) and Zika virus (ZIKV) with previous (wp) DENV infection panels. DENV1-NS1 (A) and ZIKV-NS1 (B) IgG ELISAs in convalescent-phase samples from sDENV, ZIKVwpDENV (Nicaragua [Nic]), probable ZIKVwpDENV (Brazil [Bra]) and negative control (NC) panels. C, Relative optical density (rOD) ratio of ZIKV-NS1 to DENV1-NS1. The sensitivity and specificity are shown based on a cutoff rOD ratio at 0.24. DENV1-NS1 (D) and ZIKV-NS1 (E) IgG ELISAs in post–convalescent phase samples (3 months to 2 years post–symptom onset) from sDENV panels. F, Positive rates of DENV1- and ZIKV-NS1 IgG ELISAs in sDENV panels over time. Dotted lines indicate cutoff rOD values for ELISAs. Data are mean of 2 experiments (each in duplicate). Two-tailed Mann-Whitney test was used to compare the 2 groups.

Article Snippet: Purified DENV1-NS1 protein was purchased from the Native Antigen (United Kingdom).

Techniques: Virus, Infection, Negative Control, Two Tailed Test, MANN-WHITNEY

Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

Article Title: Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests

doi: 10.1093/cid/cix672

Figure Lengend Snippet: Results of nonstructural protein 1 (NS1) immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISAs) in sequential samples from individuals with secondary dengue virus (sDENV) and Zika virus (ZIKV) infection with previous (wp) DENV infections. A, Three cases with sDENV infection, 3–18 months post–symptom onset. B, Four blood donors with sDENV infection, 7 days to 6 months post–index day (PID). C, Five blood donors with ZIKVwpDENV infection, from index day to 3 months PID. Three cases (1, 4, and 7) seroconverted to ZIKV-NS1; 2 cases (15 and 21) had ZIKV-NS1 IgG starting from the index day. Dotted lines indicate cutoff relative optical density (rOD) values for ELISAs. Data are mean of 2 experiments (each in duplicates).

Article Snippet: Purified DENV1-NS1 protein was purchased from the Native Antigen (United Kingdom).

Techniques: Enzyme-linked Immunosorbent Assay, Virus, Infection

Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

Article Title: Distinguishing Secondary Dengue Virus Infection From Zika Virus Infection With Previous Dengue by a Combination of 3 Simple Serological Tests

doi: 10.1093/cid/cix672

Figure Lengend Snippet: Proposed algorithm of using 3 serological tests (without neutralization tests) to distinguish different Zika virus (ZIKV) and dengue virus (DENV) infections in dengue- and Zika-endemic regions in the framework of Centers for Disease Control and Prevention guidelines for laboratory diagnosis of ZIKV infection [6, 7]. Only samples tested positive or equivocal by ZIKV or DENV E protein–based immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) are included in the algorithm. The total numbers from each panel and the numbers of positive or negative based on the 3 Nonstructural Protein 1 (NS1) ELISAs are shown in parentheses. Abbreviations: IgG, immunoglobulin G; OD, optical density; pDENV, primary dengue virus infection; pZIKV, primary Zika virus infection; rOD, relative OD; sDENV, secondary dengue virus infection; ZIKVwpDENV, Zika virus infection with previous dengue virus infection.

Article Snippet: Purified DENV1-NS1 protein was purchased from the Native Antigen (United Kingdom).

Techniques: Neutralization, Virus, Infection